V-FITC Apoptosis Detection Kit

Fluorescent-named protein is an integral asset for the discovery of CAR articulation in research and clinical examples by stream cytometry. These proteins are pre-marked fluorescent colors that can identify CAR articulation by one-venture staining with negligible foundation.

In any case, the improvement of excellent fluorescent-named proteins presents a significant test and there are just couple of items financially accessible up to this point.
Formation procedure is a significant bottleneck for growing top notch fluorescent-marked proteins. Right now, the most broadly involved naming strategy in the market is the conventional compound marking approach. It is an exceptionally simple technique to mark protein with a fluorescent color in a vague way covalently.

Nonetheless, arbitrary adjustment can prompt heterogeneous and may influence the bioactivity of the protein by means of obstructing the protein-dynamic site. Studies demonstrate that the new-age site-explicit marking innovation can connect fluorescent colors to explicit protein in site-explicit way. It gives an assurance to the consistency of the fluorescent-marked items and tries not to influence protein movement. This property makes it a valuable weapon for the improvement of excellent fluorescent-marked protein items with high explicitness and responsiveness.

Annexin V-FITC/PI Apoptosis Detection Kit

MCE Annexin V-FITC/PI Apoptosis Detection Kit gives a quick and advantageous technique to identify cell apoptosis and putrefaction. Subsequent to staining, live cells show practically no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence(Annexin V+/PI-), late apoptosis cells and rot cells show red and green fluorescence (Annexin V+/PI+).

Annexin V-FITC Apoptosis Detection Kit

Annexin V-FITC Apoptosis Detection Kit contains prepared to-utilize arrangements, Annexin V-FITC form, propidium iodide (PI).
The unit can recognize apoptotic and necrotic cells
Distinguish by stream cytometry or fluorescence microscopy
Don’t bother fixing cells

In typical cells, phosphatidylserines (PS, film phospholipids) are hung on the inward layer of the cell film, so Annexin V doesn’t join to the cells. During early apoptosis, the PS are uncovered on the external layer, where they append to the FITC-marked Annexin V and stain the phone surface green. During late apoptosis, propidium iodide (PI) enters the phone and stains the items red.

Apoptosis was initiated by cycloheximide (1 µg/ml) or UV light at 37°C for 3.5 hours. Cells were dissected by stream cytometry. Our item displayed about a similar presentation as the item from organization A. The control cells showed no indications of apoptosis. Treatment with cycloheximide brought about numerous cells in early apoptosis. UV light likewise instigated apoptosis in the tried cells.

Apoptosis Detection Kit Performance Evaluation

Apoptotsis was prompted by eliminating glucose and starving the cells for 48 hours. Upon investigation utilizing an imaging cytometer, a few cells were seen in early apoptosis.

Conventions
General Protocol for Suspension Cells
1. Axis the cell suspension at 1,000 rpm for 3 minutes and eliminate supernatant.
2. Add PBS to wash cells and axis at 1,000 rpm for 3 minutes, eliminate supernatant. (Do this progression two times.)
3. Add 10-crease weakened Annexin V Binding Solution to make last cell convergence of 1 x 106
cells/ml.
4. Move 100 μl of cell suspension arranged in sync 3 to another cylinder.
5. Add 5 μl of Annexin V – FITC Conjugate, then 5 μl of PI Solution to the cell suspension.
6. Hatch 15 minutes at room temperature with shield from light.
7. Add 400 μl of 10-overlay weakened Annexin V Binding Solution.
8. Apply the arrangement arranged in sync 7 to stream cytometric measure or minuscule examine.
General Protocol for Adherent Cells
1. Dispose of supernatant on the petri dish or plate.
2. Add PBS for wash cells and dispose of supernatant. (Do this progression two times.)
3. Disengage the cells with Trypsin-EDTA.
4. Add suitable volume of culture medium or PBS and move the cell suspension to a cylinder.
5. Axis at 1,000 rpm for 3 minutes. Eliminate supernatant.
6. Add PBS to wash cells and axis at 1,000 rpm for 3 minutes, eliminate supernatant. (Do this progression two times.)
7. Add 10-crease weakened Annexin V Binding Solution to make last cell convergence of 1 x 106
cells/ml.
8. Move 100 μl of cell suspension arranged at stage 7 to another cylinder.
9. Add 5 μl of Annexin V – FITC Conjugate, then, at that point, 5 μl of PI Solution to the cell suspension.

Annexin V FITC
EXB0024	ExBio	100 tests	244.8 EUR
Annexin V (FITC)
abx090611-100tests	Abbexa	100 tests	510 EUR
Annexin V, FITC Labeled
20030	AAT Bioquest	100 tests	262.8 EUR
Annexin V, FITC conjugate
29001	Biotium	0.5ML	332.4 EUR
Annexin V-FITC Reagent
1001-1000	Biovision	each	1495.2 EUR
Annexin V-FITC Reagent
1001-200	Biovision	each	529.2 EUR
Annexin A4 (ANXA4) Monoclonal Antibody (Human), FITC
4-MAE784Hu22-FITC	Cloud-Clone	367.20 EUR 3340.80 EUR 943.20 EUR 463.20 EUR 238.80 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A4 (ANXA4) Monoclonal Antibody (Pig), FITC
4-MAE784Po21-FITC	Cloud-Clone	409.20 EUR 3945.60 EUR 1094.40 EUR 523.20 EUR 258.00 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A4 (ANXA4) Monoclonal Antibody (Rat), FITC
4-MAE784Ra21-FITC	Cloud-Clone	385.20 EUR 3600.00 EUR 1008.00 EUR 489.60 EUR 247.20 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A3 (ANXA3) Monoclonal Antibody (Human), FITC
4-MAE786Hu21-FITC	Cloud-Clone	367.20 EUR 3340.80 EUR 943.20 EUR 463.20 EUR 238.80 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A3 (ANXA3) Monoclonal Antibody (Human), FITC
4-MAE786Hu23-FITC	Cloud-Clone	373.20 EUR 3427.20 EUR 964.80 EUR 471.60 EUR 242.40 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A1 (ANXA1) Monoclonal Antibody (Dog), FITC
4-MAE787Ca21-FITC	Cloud-Clone	397.20 EUR 3772.80 EUR 1051.20 EUR 506.40 EUR 252.00 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A1 (ANXA1) Monoclonal Antibody (Rat), FITC
4-MAE787Ra21-FITC	Cloud-Clone	385.20 EUR 3600.00 EUR 1008.00 EUR 489.60 EUR 247.20 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A5 (ANXA5) Polyclonal Antibody (Mouse), FITC
4-PAA259Mu01-FITC	Cloud-Clone	339.60 EUR 2942.40 EUR 843.60 EUR 423.60 EUR 226.80 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A5 (ANXA5) Polyclonal Antibody (Rat), FITC
4-PAA259Ra01-FITC	Cloud-Clone	350.40 EUR 3098.40 EUR 882.00 EUR 439.20 EUR 232.80 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A5 (ANXA5) Monoclonal Antibody (Human), FITC
4-MAA259Hu22-FITC	Cloud-Clone	346.80 EUR 3046.80 EUR 868.80 EUR 433.20 EUR 230.40 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A9 (ANXA9) Polyclonal Antibody (Human), FITC
4-PAE779Hu01-FITC	Cloud-Clone	355.20 EUR 3168.00 EUR 900.00 EUR 446.40 EUR 234.00 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A10 (ANXA10) Polyclonal Antibody (Human), FITC
4-PAE780Hu01-FITC	Cloud-Clone	355.20 EUR 3168.00 EUR 900.00 EUR 446.40 EUR 234.00 EUR	100ul 10ml 1ml 200ul 20ul
Annexin A13 (ANXA13) Polyclonal Antibody (Human), FITC
4-PAE783Hu01-FITC	Cloud-Clone	350.40 EUR 3090.00 EUR 879.60 EUR 438.00 EUR 231.60 EUR	100ul 10ml 1ml 200ul 20ul

10. Brood 15 minutes at room temperature with assurance from light.
11. Add 400 μl of 10-crease weakened Annexin V Binding Solution.
12. Apply this answer for stream cytometric examine or minute measure.