The impact of lymphocyte isolation on induced DNA damage in human blood samples measured by the comet assay.
The comet assay is incessantly utilized in human biomonitoring for the detection of publicity to genotoxic brokers. Peripheral blood samples are most incessantly used and examined both as entire blood or after isolation of lymphocytes (i.e. peripheral blood mononuclear cells, PBMC). To analyze a possible influence of lymphocyte isolation on induced DNA harm in human blood samples, we uncovered blood ex vivo to mutagens with totally different modes of genotoxic motion.
The comet assay was carried out both straight with entire blood on the finish of the publicity interval or with lymphocytes remoted straight after publicity. Along with the really helpful commonplace protocol for lymphocyte isolation, a shortened protocol was established to optimise the isolation process. The outcomes point out that the results of induced DNA strand breaks and alkali-labile websites induced by ionising radiation and alkylants, respectively, are considerably lowered in remoted lymphocytes. In distinction, oxidative DNA base harm (induced by potassium bromate) and secure cumbersome adducts (induced by benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide; BPDE) appear to be much less affected.
Our findings recommend that in vivo-induced DNA harm may also be lowered in remoted lymphocytes compared with the entire blood relying of the sorts of DNA harm induced. As a result of solely small genotoxic results can typically be anticipated in human biomonitoring research with the comet assay after occupational and environmental publicity to genotoxic brokers, any loss is perhaps related and must be averted. The potential for such results and their potential influence on variability of comet assay leads to human biomonitoring must be thought of when performing or evaluating such form of research.
Isolation and Activation of Murine Lymphocytes.
B and T cells, with their extraordinarily various antigen-receptor repertoires, have the flexibility to mount particular immune responses in opposition to virtually any invading pathogen1,2. Understandably, such intricate skills are managed by numerous molecules concerned in numerous mobile processes to make sure well timed and spatially regulated immune responses3. Right here, we describe experimental procedures that permit fast isolation of extremely purified murine lymphocytes utilizing magnetic cell sorting know-how.
The ensuing purified lymphocytes can then be subjected to varied in vitro or in vivo practical assays, such because the dedication of lymphocyte signaling capability upon stimulation by immunoblotting4 and the investigation of proliferative skills by 3H-thymidine incorporation or carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling5-7. Along with evaluating the practical capacities of management and genetically modified lymphocytes, we will additionally decide the T cell stimulatory capability of antigen-presenting cells (APCs) in vivo, as proven in our consultant outcomes utilizing transplanted CFSE-labeled OT-I T cells.
Absolute Configuration of Periplosides C and F and Isolation of Minor Spiro-orthoester Group-Containing Pregnane-type Steroidal Glycosides from Periploca sepium and Their T-Lymphocyte Proliferation Inhibitory Actions.
Additional phytochemical investigation of the foundation bark of Periploca sepium afforded 9 new spiro-orthoester group-containing pregnane-type glycosides termed periplosides O-V and 3-O-formyl-periploside A. The buildings of those glycosides together with absolutely the configuration of the distinctive seven-membered formyl acetal-bridged spiro-orthoester operate and the 4,6-dideoxy-3-O-methyl-Δ3-2-hexosulosyl moiety had been elucidated on the idea of spectroscopic knowledge interpretation and chemical transformation.
Absolutely the configurations of the main compounds periplosides C and F had been established by single-crystal X-ray diffraction evaluation. The remoted compounds had been evaluated for his or her inhibitory actions in opposition to the proliferation of T-lymphocytes. Because of this, periploside C, probably the most ample glycoside containing a spiro-orthoester moiety discovered within the plant, exhibited probably the most favourite selective index worth (SI = 82.5). The size and structure of the saccharide chain within the periplosides had been discovered to affect the inhibitory exercise and the SI worth.
Isolation of T-Cell Receptors Particularly Reactive with Mutated Tumor-Related Antigens from Tumor-Infiltrating Lymphocytes Based mostly on CD137 Expression.
Function: The adoptive switch of lymphocytes genetically modified to specific tumor reactive T-cell receptors (TCR) can mediate tumor regression. Some tumor-infiltrating lymphocytes (TIL) acknowledge somatic mutations expressed solely within the affected person’s tumors, and proof means that clinically efficient TILs goal tumor-specific neoantigens.
Right here we tried to isolate neoantigen-reactive TCRs as a prelude to the therapy of sufferers with autologous T cells genetically modified to specific such TCRs.Experimental Design: Mutations expressed by tumors had been recognized utilizing whole-exome and RNA sequencing. Tandem minigene (TMG) constructs encoding 12-24 mutated gene merchandise had been synthesized, every encoding the mutated amino acid flanked by 12 amino acids of the traditional protein sequence.
TILs had been cultured with autologous dendritic cells (DC) transfected with in vitro transcribed (IVT) mRNAs encoding TMGs and had been evaluated for IFNγ secretion and CD137 expression. Neoantigen-reactive T cells had been enriched from TILs by sorting for CD137+ CD8+ T cells and expanded in vitro Dominant TCR α and β chains had been recognized within the enriched populations utilizing a mixture of 5′ fast amplification of cDNA ends, deep sequencing of genomic DNA, PairSeq evaluation, and single-cell RT-PCR evaluation.
CancerSeq™ Plus Paraffin Tissue Tumor Sections: Thyroid |
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| T2235265-SC | Biochain | 5 curls | EUR 779 |
Matched Pair - Paraffin Tissue Sections - Human Primary Tumor and Normal (PP): Stomach |
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| T8235248-PP | Biochain | 5 slides x2 | EUR 375 |
Matched Pair - Paraffin Tissue Sections - Human Primary and Matched Metastatic Tumor (PM): Stomach |
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| T8235248-PM | Biochain | 5 slides x2 | EUR 626 |
Paraffin Tissue Section - Human Angioma |
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| T2235011 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Bone Tumor |
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| T2235023 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Lung Tumor |
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| T2235152 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Skin Tumor |
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| T2235218 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Brain Tumor |
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| T2235035 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Colon Tumor |
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| T2235090 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Liver Tumor |
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| T2235149 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Breast Tumor |
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| T2235086 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Kidney Tumor |
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| T2235142 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Testis Tumor |
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| T2235260 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Tonsil Tumor |
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| T2235268 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adipose Tumor |
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| T2235003 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adrenal Tumor |
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| T2235004 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Bladder Tumor |
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| T2235010 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Appendix Tumor |
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| T2235006 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Pancreas Tumor |
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| T2235188 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Prostate Tumor |
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| T2235201 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Abdominal Tumor |
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| T2235001 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Parathyroid Tumor |
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| T2235189 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Lung |
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| T2234152 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Skin |
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| T2234218 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Gallbladder Tumor |
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| T2235118 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain |
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| T2234035 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Colon |
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| T2234090 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart |
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| T2234122 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Liver |
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| T2234149 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Ovary |
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| T2234183 | Biochain | 5 slides | EUR 504 |
Paraffin Tissue Section - Human Adult Normal: Penis |
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| T2234194 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Angioma: Hemangioma |
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| T2235011-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Breast |
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| T2234086 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Adult Normal: Kidney |
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| T2234142 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Rectum |
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| T2234206 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Spleen |
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| T2234246 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Testis |
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| T2234260 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Throat |
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| T2234263 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Tongue |
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| T2234267 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Uterus |
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| T2234274 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Vagina |
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| T2234283 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Uterus Cervix Tumor |
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| T2235275 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Parotid |
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| T2234190 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Adipose |
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| T2234003 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Adrenal |
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| T2234004 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Bladder |
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| T2234010 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Trachea |
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| T2234160 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Stomach |
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| T2234248 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adipose Tumor: Lipoma |
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| T2235003-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Angioma: Glomus Tumor |
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| T2235011-2 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Bone Tumor: Chondroma |
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| T2235023-2 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - NASH: Liver |
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| T2236149NAS | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Lupus: Lung |
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| T2236152Lup | Biochain | 5 slides | EUR 243 |
Paraffin Tissue Section - Human Adult Normal: Appendix |
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| T2234006 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Pancreas |
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| T2234188 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Placenta |
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| T2234200 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Prostate |
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| T2234201 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adrenal Tumor: Adenoma |
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| T2235004-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Brain Tumor: Epndymoma |
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| T2235035-6 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Testis Tumor: Seminoma |
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| T2235260-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Lupus: Heart |
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| T2236122Lup | Biochain | 5 slides | EUR 243 |
Paraffin Tissue Section - Lupus: Liver |
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| T2236149Lup | Biochain | 5 slides | EUR 243 |
Paraffin Tissue Section - Asthma: Lung |
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| T2236152Ld-1 | Biochain | 5 slides | EUR 475 |
Paraffin Tissue Section - Human Tumor Cell Line: MCF-7 |
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| T2255830 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Parathyroid |
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| T2234189 | Biochain | 5 slides | EUR 629 |
Paraffin Tissue Section - Human Adult Normal: Esophagus |
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| T2234106 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Diaphragm |
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| T2234169 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Brain Tumor: Gliocytoma |
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| T2235035-7 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Brain Tumor: Meningioma |
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| T2235035-9 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Lupus: Kidney |
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| T2236142Lup | Biochain | 5 slides | EUR 243 |
Paraffin Tissue Section - Lupus: Spleen |
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| T2236246Lup | Biochain | 5 slides | EUR 243 |
Paraffin Tissue Section - Human Adult Normal: Brain: Pons |
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| T2234071 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Eye: Retina |
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| T2234110 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Adult Normal: Lymph Node |
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| T2234161 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Epididymis |
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| T2234105 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Skin Tumor: Fibrosarcoma |
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| T2235218-2 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Spinal Cord |
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| T2234234 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Kidney Tumor: Wilms' Tumor |
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| T2235142-7 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Gallbladder |
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| T2234118 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Emphysema: Lung |
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| T2236152Ld-3 | Biochain | 5 slides | EUR 475 |
Paraffin Tissue Section - Pneumonia: Lung |
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| T2236152Ld-4 | Biochain | 5 slides | EUR 475 |
Paraffin Tissue Section - Lupus: Pancreas |
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| T2236188Lup | Biochain | 5 slides | EUR 243 |
Paraffin Tissue Section - Human Adult Normal: Brain: Insula |
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| T2234055 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adrenal Tumor: Myelolipoma |
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| T2235004-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Bone Tumor: Chondrosarcoma |
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| T2235023-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Breast Tumor: Fibroadenoma |
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| T2235086-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Lung Tumor: Adenocarcinoma |
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| T2235152-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Bronchitis: Lung |
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| T2236152Ld-2 | Biochain | 5 slides | EUR 475 |
Paraffin Tissue Section - Human Colon Tumor: Adenocarcinoma |
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| T2235090-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Liver Tumor: Adenocarcinoma |
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| T2235149-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain: Amygdala |
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| T2234036 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Adult Normal: Brain: Midbrain |
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| T2234059 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Brain: Thalamus |
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| T2234079 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Bone Tumor: Synovial Sarcoma |
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| T2235023-8 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain: Pituitary |
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| T2234068 | Biochain | 5 slides | EUR 758 |
Paraffin Tissue Section - Human Adult Normal: Ductus Deferens |
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| T2234100 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Adult Normal: Skeletal Muscle |
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| T2234171 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Small Intestine |
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| T2234226 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Brain Tumor: Ependymal Glioma |
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| T2235035-5 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Angioma: Cavernous Hemangioma |
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| T2235011-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - hypertension: Heart |
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| T2236122Hd-2 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain: Cerebellum |
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| T2234039 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Skin Tumor: Malignant Melanoma |
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| T2235218-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Diabetic Diseased Tissue: Lung |
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| T2236152Dia | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Appendix Tumor: Adenocarcinoma |
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| T2235006-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Brain Tumor: Craniopharyngioma |
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| T2235035-4 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Pancreas Tumor: Adenocarcinoma |
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| T2235188-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Prostate Tumor: Adenocarcinoma |
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| T2235201-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Blood Vessel: Vein |
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| T2234020 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Brain: Optic Nerve |
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| T2234064 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Adult Normal: Heart: Aorta Valve |
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| T2234123 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart: Atrium (left) |
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| T2234126 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Brain: Hippocampus |
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| T2234052 | Biochain | 5 slides | EUR 629 |
Paraffin Tissue Section - Human Adult Normal: Heart: Pericardium |
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| T2234133 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Bone Tumor: Myeloma, Plasmacytic |
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| T2235023-7 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Brain Tumor: Meningioma (Fibrous) |
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| T2235035-10 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Breast Tumor: Lobular carcinoma |
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| T2235086-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Colon Tumor: Adenocarcinoma, MSH |
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| T2235090MSH | Biochain | 5 slides | EUR 734 |
Paraffin Tissue Section - Human Colon Tumor: Adenocarcinoma, MSS |
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| T2235090MSS | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Human Diabetic Diseased Tissue: Colon |
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| T2236090Dia | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Diabetic Diseased Tissue: Liver |
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| T2236149Dia | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain: Frontal Lobe |
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| T2234051 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Brain: Pineal Gland |
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| T2234067 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Adult Normal: Heart: Arcus Aortae |
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| T2234124 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart: Atrium (right) |
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| T2234127 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart: Mitral Valve |
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| T2234131 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Lung Tumor: Large Cell Carcinoma |
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| T2235152-6 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Lung Tumor: Small Cell Carcinoma |
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| T2235152-9 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Pancreas Tumor: Islet Cell Tumor |
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| T2235188-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Tonsil Tumor: Lymphoma of Tonsil |
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| T2235268-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain: Parietal Lobe |
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| T2234066 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Liver Cirrhosis: Lung |
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| T2236152Lcs | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain: Diencephalon |
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| T2234049 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Submaxillary gland |
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| T2234165 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Brain Tumor: Meningioma (Necrotic) |
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| T2235035-11 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section – Human Breast Tumor, BRCA Characterized |
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| T2235086BRCA | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Human Rectum Tumor: Adenocarcinoma, MSS |
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| T2235206MSS | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Human Uterus Tumor: Adenocarcinoma, MSS |
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| T2235274MSS | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Human Adult Normal: Blood Vessel: Artery |
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| T2234013 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Brain: Temporal Lobe |
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| T2234078 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart: Auricula (left) |
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| T2234128 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Brain Tumor: Astrocytoma Grade I |
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| T2235035-1 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Brain Tumor: Astrocytoma Grade II |
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| T2235035-2 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV positive |
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| T2235275HPV+ | Biochain | 5 slides | EUR 646.8 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative |
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| T2235275HPV- | Biochain | 5 slides | EUR 646.8 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative |
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| T2235275HPV-negative | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV positive |
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| T2235275HPV-positive | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Liver Cirrhosis: Heart |
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| T2236122Lcs | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Liver Cirrhosis: Liver |
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| T2236149Lcs | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Colon Tumor: Adenocarcinoma (Ulcer) |
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| T2235090-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Stomach Tumor: Adenocarcinoma, MSS |
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| T2235248MSS | Biochain | 5 slides | EUR 524 |
Paraffin Tissue Section - Human Diabetic Diseased Tissue: Stomach |
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| T2236248Dia | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Parotid Tumor: Pleomorphic Adenoma |
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| T2235190-5 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Abdominal Tumor: Neuofibromatosis |
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| T2235001-7 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Adult Normal: Brain: Choroid Plexus |
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| T2234044 | Biochain | 5 slides | EUR 330 |
Paraffin Tissue Section - Human Adult Normal: Brain: Occipital Lobe |
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| T2234062 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart: Auricula (right) |
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| T2234129 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart: Papilla Muscle |
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| T2234132 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Adult Normal: Heart: Ventricle (left) |
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| T2234138 | Biochain | 5 slides | EUR 185 |
Paraffin Tissue Section - Human Brain Tumor: Astrocytoma Grade III |
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| T2235035-3 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Kidney Tumor: Clear cell carcinoma |
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| T2235142-2 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Kidney Tumor: Renal Cell Carcinoma |
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| T2235142-5 | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Diabetic Diseased Tissue: Parathyroid |
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| T2236189Dia | Biochain | 5 slides | EUR 629 |
Paraffin Tissue Section - Liver Cirrhosis: Kidney |
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| T2236142Lcs | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Liver Cirrhosis: Spleen |
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| T2236246Lcs | Biochain | 5 slides | EUR 238 |
Paraffin Tissue Section - Human Brain Tumor: Pilocytic Astrocytoma |
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| T2235035-13 | Biochain | 5 slides | EUR 238 |
Human PBL retrovirally transduced to specific the TCRs had been evaluated for recognition of related neoantigens.Outcomes: We recognized 27 TCRs from 6 sufferers that acknowledged 14 neoantigens expressed by autologous tumor cells.Conclusions: This technique offers the means to generate T cells expressing neoantigen-reactive TCRs to be used in future adoptive cell switch immunotherapy trials for sufferers with most cancers.