For the quantitative determination of phosphate and evaluation of the effects of drugs on phosphate metabolism.
- Sensitive and precise. Linear detection range 0.3 μM (0.0028 mg / dL) to 50 μM (0.47 mg / dL) of phosphate in 96-well plate assay.
- Simple and high performance. The procedure involves the addition of a single working reagent and incubation for 30 min. It can be easily automated as a high throughput assay for thousands of samples per day.
- Greater stability and versatility of reagents. The optimized formulation has greatly improved signal and reagent stability. Assays can be performed in a 96-well plate or cuvette.
- Low interference in biological samples. No pretreatments are needed. Assays can be performed directly on raw biological samples, that is, in the presence of lipids, proteins, and minerals.
OD620nm (malachite green)
Serum, urine, saliva, sweat, tissue culture, food, environment, etc.
Size: 500 tests
Detection limit: 3 μg / dL (0.3 μM)
Duration: 12 months
Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is an important component of minerals and fertilizers, and it is a component of industrial wastewater. Therefore, accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring, and environmental monitoring. Simple, straightforward, automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular.
The BioAssay Systems Phosphate Assay Kit is designed to measure phosphate ions directly in samples without any pretreatment. The improved malachite green method uses the malachite green molybdate dye, which forms a stable color complex specifically with inorganic phosphate. The intensity of the color, measured at 620 nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces substance interference in raw samples.