PDQ Protease Assay.

N-acetyl-D-glucosamine (GlcNAc) is phosphorylated by the chemical GlcNAc Kinase (NagK) and ATP to shape GlcNAc-6-phosphate and ADP. Involving a coupled response within the sight of NADH, how much NAD framed is stoichiometric with how much GlcNAc, and is estimated by a reduction in absorbance at 340 nm.

The PDQ Protease Assay is a novel colorimetric measure used to distinguish protease movement in fluid examples. The restrictive substrate answers a wide scope of proteases including serine, metallo, aspartate and cysteine proteases like collagenase, proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin.

PDQ can be utilized with only a couple of basic strides to quantify protease action and requires no centrifugation. The substrate is a cross-connected framework containing protein substrate and a color protein form. Protease movement is identified spectrophotometrically with expanding optical thickness relative to expanding chemical action and can recognize nanogram amounts. Each pack is provided with a trypsin answer for creating standard bends (BAEE identical units). PDQ is provided in 48 prepared to-utilize plastic vials.

The diagram on the right shows the time-course debasement of the PDQ Protease Assay substrate by papain, chymotrypsin, proteinase K, and collagenase. Copy responses were brooded at 37°C and 0.2 N NaOH was added to the vials to stop the response at the demonstrated times. The absorbance at 450nm was estimated by moving the response combination to spectrophotometric cuvettes. The aftereffects of this investigation show the expanded assimilation over the long haul due to protelytic corruption of the substrate, and the capacity of the examine to recognize a wide scope of proteases.

Specialized Considerations.

1. Cushions that can be utilized to plan test tests
may go in pH from 4 to 10.
2. How much time expected for brooding can
be changed to upgrade the measure. Up to 24 hr
hatching periods are conceivable which will build the
recognition cutoff points of the examine. Then again, for
applications with high measures of chemical, 15
min might do the trick. The specific circumstances ought to be
decided exactly.
3. Similarly as with any examine, running a standard is fundamental
bend with control protease each time the measure is
performed with obscure protease tests.
4. While utilizing the vials, the halted response
contents should be moved to a cuvette to
measure the absorbance. The exchange of the
contents from the PDQ framework ought to be
done as quickly as time permits after the expansion of
NaOH, since the NaOH will debase the framework
over the long run. Be that as it may, the items can be left
on the lattice for as long as one hour before move
without compromising the exactness of the test.
5. When the halted response contents are
moved away from lattice, they can be left for
up to 24 hrs covered (to keep out light) at room
temperature prior to estimating the absorbance
without loss of sign.
6. To involve the Colorimetric Assay for misty
tests, run the response as portrayed. Eliminate
the response fluid and afterward back digest the
remaining substrate network with Trypsin, Subtilisin
or then again Proteanase K. The sign produced from the
back assimilation is conversely corresponding to the
measure of compound action.

AthenaES inc.

Athena Enzyme Systems is a division of Athena Environmental Sciences, Inc.. Our gathering gives items and administrations pointed toward assisting specialists and makers with working on the creation and recuperation of recombinant proteins.

The Athena Enzyme Systems bunch fabricates and sells items which include: a restrictive line of bacteriological media explicitly intended to build the outflow of recombinant proteins; a line of sans serum mode for the way of life of human cell lines alongside extra items for cell culture; the PDQ Protease Assay, an expansive range, one stage protease measure; specialty catalysts, for example, the AFM reference protein I27O as well as bacterial esterases and different proteins; and a line of reagents for the refolding of denatured proteins. Each of our items are made with consideration regarding quality, dependability, and convenience.

Athena Enzyme Systems additionally gives life-cycle contract exploration and assembling administrations to industry, government, and scholarly clients for the creation and filtration of recombinant and local proteins.

Our turn of events and creation groups can deliver and sanitize from milligram to gram amounts of protein. The extent of accessible administrations includes quality cloning, strain development, articulation advancement, improvement of decontamination plans, and creation of the item.

Each venture is altered to address the issues of the client. The organization’s cutting edge research center and creation offices are completely prepared for sub-atomic science, microbiological, immunochemical, and protein natural chemistry projects. Athena’s staff gets mastery atomic science, protein natural chemistry, microbial science, enzymology, immunochemistry, toxicology, logical science, materials science, and biotech fabricating.

Our parent organization, Athena Environmental Sciences, Inc., is a broadened biotechnology organization established in 1994 and situated in Baltimore, MD.

AthenaES represents considerable authority in the turn of events and commercialization of cutting edge biotechnology-based items and gives life cycle contract R&D and fabricating administrations for advancement of imaginative thoughts from conceptualization to creation of item. The organization makes a few different ecologically mindful items under the Athena Environmental Sciences mark as well as under private names for different organizations.

Our gathering’s maxim, “straightforward answers for complex proteins” typifies our way of thinking of furnishing you with dependable answers for your protein creation needs. We endeavor to make our results of the greatest quality and back their exhibition with certifiable applications testing.


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A significant number of our items have been being used by fundamental researchers and in business activities for more than 10 years. As a component of our obligation to bring the biotechnology local area valuable instruments, on the off chance that we don’t convey an item you want, if it’s not too much trouble, let us know and we’ll do everything possible to make it.

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