Step 1. Korean tiger whole-genome sequencing
Tiger Genome Project has started in March 2010, followed by consultations with Tiger Preservation Association, SangMyung University, and National Science Museum in summer 2010. The whole-genome sequencing process has begun in November 2010, with the collected blood sample of a Siberian tiger in Everland. DNA is extracted from the tiger’s blood and it is randomly amplified to produce large-quantity genetic information. Afterward, the genetic information is aligned in order to sequence the genome.
Step 2. Korean tiger genome analysis
Large-quantity parallel sequence analysis describes the genome sequence of a tiger, and the annotation and a number of analysis techniques analyze the genetic information of the whole genome by predicting the genetic functions. This marks the completion of the Tiger Genome Project.
Step 3. Genome analysis of all species of tiger
Genome analysis of one tiger subspecies does not fully represent all tiger subspecies’ genetic traits. Therefore, samples of extinct tigers are procured to additionally analyze a total of 9 tiger subspecies’ genomes. This completes the Tiger Genome Project and will provide solutions in preserving the tiger and studying its evolutionary process.
Step 4. Genome analysis of all big cat species
Genome analysis of tiger species does not fully represent all big cat species’ genetic traits. Therefore, samples of other big cats are procured to analyze them. This completes the Big Cat Genome Project and will provide solutions in preserving the big cats.